|
NR 17/2014
|
Recognition of replacement methods to the use of animals in research activities in Brazil |
Description of the methods |
|
NR 18/2014
|
Skin irritation and corrosion |
OECD GT 430 - In vitro dermal corrosion (transcutaneous dermal resistance test) |
| OECD GT 431 - In vitro dermal corrosion (reconstituted human epidermis test) |
| OECD GT 435 – In vitro membrane barrier test |
| OECD GT 439 – In vitro membrane barrier test |
| Eye irritation and corrosion |
OECD GT 437 - Bovine cornea permeability and opacity test |
| OECD GT 438 - Isolated chicken eye test |
| OECD GT 460 - Fluorescein permeation test |
| Phototoxicity |
OECD GT 432 - In vitro phototoxicity test 3T3 NRU |
| Skin absorption |
OECD GT 428 - Cutaneous absorption (in vitro method) |
| Potential for skin sensitization |
OECD GT 429 - Cutaneous sensitization (local lymph node assay) |
| OECD GT 442A and 442B - Non-radioactive versions of the local lymph node assay |
| Acute toxicity |
OECD GT 420 - Acute oral toxicity – Fixed dose procedure |
| OECD GT 423 - Acute oral toxicity – Acute toxic class |
| OECD GT 425 - Acute oral toxicity – Up and Down procedure |
| OECD GT 129 - Estimation of the initial dose for systemic oral acute toxicity testing |
| Genotoxicity |
OECD GT 487 - Micronucleus test in mammalian cells in vitro |
|
NR 31/2016
|
Eye irritation and corrosion |
OECD GT 491 – Short-term in vitro test for eye damage |
| OECD GT 492 – Reconstituted human corneal epithelium |
| Skin sensitization |
OECD GT 442C – In chemico skin sensitization |
| OECD GT 442– In vitro skin sensitization |
| Reproductive toxicity |
OECD GT 421 - Screening test for reproductive and developmental toxicity |
| OECD GT 422 – Repeated toxicity study combined with reproductive toxicity test |
| Assessment of pyrogenic contamination in injectable products |
Bacterial endotoxin test (Brazilian Pharmacopoeia) |
| NR 45/2019 |
Assessment of pyrogenic contamination in injectable products |
OECD GT 34 – Monocyte activation test |
| NR 54/2022 |
Recognition of alternative methods for teaching and scientific research activities (repealed NR 17) |
| NR 56/2022 |
Dermal sensitization |
OECD GT 442E – In vitro skin sensitization |
| Estrogenic effects OECD GT 493 – In vitro human estrogen receptor (hrER) assays to detect chemicals with ER binding affinity |
OECD GT 455 – In vitro transactivation assays to detect agonists and antagonists of estrogen receptors |
| |
| Endocrine effects |
OECD GT 456 – Steroidogenesis assay |
| Androgen effects |
OECD GT 458 – Transcriptional activation of transfected human androgen receptors for detection of agonist and antagonist activity of chemicals |
| Mutagenicity |
OECD GT 471 – Bacterial reverse mutation test |
| OECD GT 473 – In vitro mammalian chromosomal aberration test |
| OECD GT 476 – In vitro gene mutation testing of mammalian cells using the Hprt and xprt genes |
| OECD GT 490 – In vitro gene mutation tests in mammalian cells using Thymidine kinase gene |
| Eye irritation/corrosion |
OECD TG 494 – Eye irritation testing to identify chemicals which do not require classification and labeling for eye irritation or serious eye damage. |
| OECD GT 496 – In vitro testing to identify chemicals that induce severe eye damage and chemicals which do not require classification for eye irritation or severe eye damage. |
| Photoreactivity |
OECD GT 495 – Photoreaction assay by reactive oxygen species |
| OECD GT 212 – Fish, short-term toxicity testing in embryonic and newborn stages |
| OECD GT 236 – Acute fish embryo toxicity (FET) |
| OECD GT 319-A – Determination of intrinsic in vitro clearance using cryopreserved Rainbow Trout hepatocytes (RT-HEP) |
| OECD GT 319-B - Determination of intrinsic clearance in vitro using sub-cellular fraction S-9 of Rainbow Trout (RT-S9) |
A brief history of the animals’ scientific research, international rules and Brazilian regulations
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