Two new species of the industrially relevant genus <i>Absidia</i> (Mucorales) from soil of the Brazilian Atlantic Forest

Cordeiro, Thalline Rafhaella Leite; Nguyen, Thuong Thuong Thi; Lima, Diogo Xavier; Silva, Suzana Brito Gomes da; Lima, Catarina Ferreira de; Leitão, Joana D’Arc Alves; Gurgel, Luciana Melo Sartori; Lee, Hyang Burm; Santiago, André Luiz Cabral Monteiro de Azevedo

doi:10.1590/0102-33062020abb0040

Thalline Rafhaella Leite Cordeiro

Programa de Pós-Graduação em Biologia de Fungos, Departamento de Micologia, Universidade Federal de Pernambuco, 50670-420, Recife, PE, Brazil

https://orcid.org/0000-0002-3430-5852

Thuong Thuong Thi Nguyen

Environmental Microbiology Lab, Department of Agricultural Biological Chemistry, College of Agriculture and Life Sciences, Chonnam National University, 61186, Gwangju, Korea

https://orcid.org/0000-0002-3300-9226

Diogo Xavier Lima

Programa de Pós-Graduação em Biologia de Fungos, Departamento de Micologia, Universidade Federal de Pernambuco, 50670-420, Recife, PE, Brazil

https://orcid.org/0000-0001-7951-8934

Suzana Brito Gomes da Silva

Programa de Pós-Graduação em Biologia de Fungos, Departamento de Micologia, Universidade Federal de Pernambuco, 50670-420, Recife, PE, Brazil

https://orcid.org/0000-0002-7514-0994

Catarina Ferreira de Lima

Programa de Pós-Graduação em Biologia de Fungos, Departamento de Micologia, Universidade Federal de Pernambuco, 50670-420, Recife, PE, Brazil

https://orcid.org/0000-0002-4882-5808

Joana D’Arc Alves Leitão

Programa de Pós-Graduação em Biologia de Fungos, Departamento de Micologia, Universidade Federal de Pernambuco, 50670-420, Recife, PE, Brazil

https://orcid.org/0000-0002-0631-1462

Luciana Melo Sartori Gurgel

Instituto Agronômico de Pernambuco, 50761-000, Recife, PE, Brazil

https://orcid.org/0000-0002-7431-2889

Hyang Burm Lee

Environmental Microbiology Lab, Department of Agricultural Biological Chemistry, College of Agriculture and Life Sciences, Chonnam National University, 61186, Gwangju, Korea

https://orcid.org/0000-0001-5353-2586

André Luiz Cabral Monteiro de Azevedo Santiago ^** Corresponding author: andrelcabral@msn.com

Programa de Pós-Graduação em Biologia de Fungos, Departamento de Micologia, Universidade Federal de Pernambuco, 50670-420, Recife, PE, Brazil

https://orcid.org/0000-0001-5174-5553

* Corresponding author: andrelcabral@msn.com

Species name	Collection No.	GenBank accession No.
Species name	Collection No.	ITS	LSU
Absidia anomala Hesselt. & J.J. Ellis	¹CBS 125.68^T⁵FSU5798	JN205815 EF030523	JN206593 -
Absidia caatinguensis D.X. Lima & A.L. Santiago	⁶URM7156^T	KT308168	KT308170
Absidia californica J.J. Ellis & Hesselt.	¹CBS 314.78	JN205816	JN206582
Absidia coerulea Bainier	¹CBS 102.28 ¹CBS 104.08	JN205821 JN205811	JN206584 HM849703
Absidia cuneospora G.F. Orr & Plunkett	¹CBS 101.59^T¹CBS 102.59	- JN205819	JN206580 JN206579
Absidia cylindrospora var. cylindrospora Hagem	¹CBS 100.08	JN205822	JN206588
Absidia cylindrospora var. nigra Hesselt. & J.J. Ellis	¹CBS 127.68^T	-	JN206589
Absidia cylindrospora var. rhizomorpha Hesselt. & J.J. Ellis	¹CBS 153.63^T	-	JN206594
Absidia fusca Linnem.	¹CBS 102.35^T¹CBS 346.97	JN205814 JN205817	HM849707 -
Absidia glauca Hagem	¹CBS 101.08 ¹CBS 100. 48	JN205810 JN205820	HM849705 JN206581
Absidia heterospora Y. Ling	¹CBS101.29^T	-	JN206595
Absidia jindoensis Hyang B. Lee & T.T.T. Nguyen	²CNUFC-PTI1-1^T	MF926622	MF926616
Absidia koreana Hyang B. Lee, Hye W. Lee & T.T. Nguyen	³EML-IFS45-1^T³EML-IFS45-2	KR030062 KR030063	KR030056 KR030057
Absidia macrospora Váňová	⁵FSU4746 ¹CBS 697.68^T	AY944882 -	EU736303 HM849704
Absidia multispora sp. nov. T.R.L. Cordeiro, D.X. Lima, Hyang B. Lee & A.L. Santiago	⁶ URM 8210 ^T	MN953780	MN953782
Absidia pseudocylindrospora Hesselt. & J.J. Ellis	¹CBS 100.62^T	NR_145276	JN206591
Absidia psychrophilia Hesselt. & J.J. Ellis	¹CBS 17268^T	-	JN206587
Absidia repens Tiegh.	¹CBS 115583	JN205813	HM849706
Absidia saloaensis sp. nov. T.R.L. Cordeiro, D.X. Lima, Hyang B. Lee & A.L. Santiago	⁶ URM 8209 ^T	MN953781	MN953783
Absidia spinosa var. spinosa Lendn.	¹CBS 106.08	JN205809	JN206590
Absidia terrestris Rosas de Paz, Dania García, Guarro, Cano & Stchigel	⁴FMR 14989^T	LT795003	LT95005
Chlamydoabsidia padenii Hesselt. & J.J. Ellis	¹CBS 172.67^T	-	JN206586
Cunninghamella phaeospora Boedijn	¹CBS 692.68^NT	JN205864	HM849697
Cunninghamella vesiculosa P.C. Misra	¹CBS 989.96^T	JN205897	HM849693
Halteromyces radiatus Shipton & Schipper	¹CBS 162.75^T	-	JN206596

Species	Colony color	Whorls	Sporangiophores	Sporangia	Columellae	Projection	Sporangiospores
A. anomala ³	Gray to light violet.	Up to 2.	40-105 × 3-9 µm.	Pyriform, 12-26 µm in diam.	Hemispherical, 6.5-20 µm in diam.	Up to 2 µm in length.	Cylindrical to somewhat constricted in the center to short oval, 3-4 × 2.2 µm.
A. californica ⁴	Light gray to grayish olive.	Not formed.	150-250 × 7-11 µm.	Globose to oval, 10-30 µm in diam.	Hemispherical, some hemielliptical, 14.5-26.5 µm in diam.	Up to 4.5 × 5.5 µm.	Globose, 3.5-4 µm in diam.
A. cuneospora ¹	Light gray to pearl gray.	Up to 3.	30-150 × 2-4 µm.	Globose, 13-32 μm in diam.	Hemispherical to nearly globose, 9-19.5 μm in diam.	Swollen at apex, 6-7 × 2.5-3 μm.	Lacrimoid to wedge-shaped, 4.5-6.5 µm in length.
A. cylindrospora ³	Pale olive-buff.	Up to 4.	36-300 × 2-7 µm.	Pyriform, 10-35 μm diam.	Hemispherical, 8.5-26 µm diam.	Rounded and often bulbous, up to 4.5 µm in length.	Cylindrical to slightly broader at one end, 3.3-5.5 × 2.2-3.5 µm.
A. caatinguensis ⁶	Brownish gray.	Up to 6 (7).	40-150 × 2.5-5 µm.	Pyriform 17.5-27.5 μm diam.	Mostly hemispherical, some subglobose, 10-20 μm diam.	Bulbous at distal end up to 5.75 × 2.5 μm.	Cylindrical, slightly constricted at the central portion, 5-7.5 × 2.5-3.7 μm.
A. koreana ⁶	Grayish with or smoky gray.	Up to 6.	3.84-4.6 μm in width, variable in length.	Globose to slightly elliptical, 19.33-23.64 × 21.06-26.3 μm.	Globose, 10.9-16.96 × 11.46-18.89 μm.	Not informed.	Short-cylindrical or cylindrical, 3.54-4.48 × 2.15-2.35 μm.
A. multispora sp. nov.⁸	Brownish gray, turning dark gray.	Up to 2 (4).	Up to 270 × 5 µm.	Pyriform and subglobose, up to 30 µm in diam.	Hemispherical, subglobose, or strawberry-shaped, 10-16 × 10-15 µm in diam.	Mostly conical, or elongated, needle-like, up to 5 × 2.5 µm.	Globose, subglobose 5-7.5 diam, ellipsoid, short cylindrical, broadly-ellipsoidal, irregular, 5-9.5 × 5-7.5 µm.
A. pseudocylindrospora ^2,3	Pale to dark olive-gray	Up to 5-7 (11).	45-172 × 3-6 µm.	Pyriform, 15-35 µm in diam.	Globose to nearly hemispherical, 9-26 µm in diam.	Globose to hemispherical, up to 6 µm in length.	Cylindrical or nearly so, up to 3.5-5 × 2.4 µm.
A. repens ⁵	Light grayish olive to olive gray.	Not formed.	140-250 × 2.5-6 µm; short sporangiophores 12-78 × 2.2-4.5 µm.	Oval to elliptical, 15-36 × 7-15 µm, turning globose, 19-26.5 µm in diam.	Hemispherical, 5-25 µm in diam.	With a bulbous swelling at end, up to 9 µm in length.	Short oval to irregular oval, 2.8-5.5 × 2-3 µm, few globose up to 6.5 µm in diam,
A. saloaensis sp. nov.⁸	Grayish brown.	Up to 5 (6).	Up to 280 × 6 µm.	Pyriform, 20-35 µm in diam.	Conical to subglobose and strawberry-shaped, 7-22 × 8.5-20 µm.	Elliptical, conical, or needle-shaped, up to 5 × 3.5 µm.	Cylindrical and elliptical, 5-7 × 2.5-3.5 µm, some slightly constricted in the center.
A. spinosa ³	Smoke gray to drab.	Up to 1-4 (8).	100-250 × 5-10.5 µm.	Pyriform, up to 12-30 µm in diam.	Hemispherical, 8-21 µm in diam.	Cylindrical to rounded, up to 1.5-4.5 × 0.5-1 µm.	Short cylindrical with rounded ends, up to 5 × 5.5 µm.
A. terrestris ⁷	Grayish brown.	Not formed.	25-215 × 2.5-5 µm.	Pyriform, 17.5-27.5 × 17.5-22.5 µm.	Globose, 5-7.5 µm diam.	Up to 5-7.5 µm.	Cylindrical, 4-5 × 2-4 µm.

[TFN1] ¹
CBS culture collection of the Westerdijk Fungal Biodiversity Institute, The Netherlands; ² CNUFC: Chonnam National University Fungal Collection,Gwangju, South Korea; ³ EML: Environmental Microbiology Laboratory (Fungarium, Chonnam National University), Gwangju, South Korea; ⁴ FMR: Universitat Rovira i Virgili (URV), Tarragona, Spain; ⁵ FSU: Friedrich Schiller University, Jena, Germany; ⁶ URM Micoteca and URM herbarium: Universidade Federal de Pernambuco, Recife, Brazil ; ^T and ^NT = ex-type and ex-neotype strains.

Two new species of the industrially relevant genus Absidia (Mucorales) from soil of the Brazilian Atlantic Forest

ABSTRACT