Open-access Molecular characterization of Kappa-casein and β-lactoglobulin genes in Anatolian Black cattle and Holstein breeds

[Caracterização molecular dos genes Kappa-casein e β-lactoglobulina em gado negro anatoliano e raças Holstein]

ABSTRACT

κ-Cn and β-lactoglobulin are important candidate genes associated with milk yield and milk protein content. The present investigation is carried out to determine the polymorphisms status of κ-Cn and β-lactoglobulin genes in Anatolian Black cattle and Holstein breeds. PCR-RFLP technique was used to determine Kappa-Casein and β-lactoglobulin polymorphisms in both cattle breeds. The allele frequency of Anatolian Black cattle in terms of κ-Cn and β-lactoglobulin genes were 0.50 (A) 0.50 (B) and 0.20 (A) 0.80 (B) respectively, whereas in Holstein were 0.29 (A) 0.71 (B) and 0.44 (A) 0.56 (B) respectively. The chi-square test showed that each cattle breed was in Hardy-Weinberg equilibrium (P>0.05).

Keywords: κ-Cn; β-lactoglobulin; Anatolian Black; Holstein; polymorphism

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Universidade Federal de Minas Gerais, Escola de Veterinária Caixa Postal 567, 30123-970 Belo Horizonte MG - Brazil, Tel.: (55 31) 3409-2041, Tel.: (55 31) 3409-2042 - Belo Horizonte - MG - Brazil
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