Gastric content technique collection for <i>in vitro</i> degradation and gas production in horses supplemented with live yeast and protected yeast

Pombo, G.V.; Pereira, Y.S.; Mazzo, H.C.; Franzan, B.C.; Pimentel, V.S.; Alves, G.E.S.; Faleiros, R.R.; Oliveira, A.P.L.; Paz, C.F.R.; Gobesso, A.A.O.

doi:10.1590/1678-4162-13054

home Sumário navigate_before Anterior Atual Seguinte navigate_next

home Sumário

Original Article - Zootechnics • Arq. Bras. Med. Vet. Zootec. 76 (4) • Jul-Aug 2024 • https://doi.org/10.1590/1678-4162-13054 linkcopy

Gastric content technique collection for in vitro degradation and gas production in horses supplemented with live yeast and protected yeast

[Técnica de coleta de conteúdo gástrico para degradação e produção de gases in vitro de equinos suplementados com levedura viva e levedura viva protegida]

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

Due to the difficulty in acessing certain sites of fermentation and possible starch digestion, studies show the need for equine gastric digestive fisiology evaluation. The aim of this experiment was to assess horses supplemented with live yeast and protected live yeast (Saccharomyces cerevisae NCYC Sc 47) to validate a new procedure of gastric content collection for determination of pH, latic acid, short chain fatty acids and gas production and degradation to be used in further in vitro fermentative studies. The experimental design used was a the latin square (4x4) for 4 periods and 1 day of gastric content collection, with 15 days of rest between it. The groups were divided in control (CTRL), non protected live yeast (NPYEA), protected live yeast (PYEA) and a combination of live yeast + protected yeast (COMB). Treatment means were compared using orthogonal contrasts (C1: CTRL versus NPYEA, PYEA and COMB; C2: COMB versus NPYEA and PYEA; C3: NPYEA versus PYEA) and Tukey´s test was used at a 5% significance level. The procedure was highly tolerated and provided great accuracy of the collection site. The live yeast supplementation increased the concentration of acetic acid at the horses’ stomachs. When gastric content was used as inoculum for in vitro fermentation, the protected live yeast produced less gas.

Keywords: degradation; equine; fermentation; nutraceutical; stomach

Nutrients	Hay	Control Concentrate	Protected live yeast concentrate
Dry Matter (DM)	91.12	89.00	89.00
Crude Protein (CP)	20.60	15.00	14.50
Ether Extract (EE)	2.26	7.30	7.00
Neutral Detergent Fiber (NDF)	70.20	25.00	26.00
Acid Detergent Fiber (ADF)	48.30	9.30	12.40
Energy digestive (ED: Mcal/kg)	2.20	3.55	3.38

Itens (mmol/L)	Treatments				SEM	P- value
Itens (mmol/L)	CTRL	NPYEA	PYEA	COMB		Treat	C1	C2	C3
Acetic. Ac.	3.15^c	4.37^bc	5.27^ab	6.36^a	0.3538	0.0082*	0.0062	0.044	0.2899
Ac. Pr.	1.59	1.55	1.60	1.66	0.0546	0.8712	0.4831	0.4904	0.7013
Ac. Isop.	-	-	-	-	-	-	-	-	-
Ac. But	-	-	-	-	-	-	-	-	-
Ac. Isob.	-	-	-	-	-	-	-	-	-
Ac. Val.	-	-	-	-	-	-	-	-	-
Ac. Lat.	0.30	0.90	0.73	0.22	0.6029	0.5953
pH	6.2	6.4	6.1	6.3	0.2409	0.8420

Item	Treatments				SEM	P-value
Item	CTRL	NPYEA	PYEA	COMB
A (mL. g ^-1 MS)	5685	1309	12571	1015	258.96	0.4916
b (h ^-1 )	0.1467^b	0.2385^ab	0.1216^b	0.3227^a	0.0216	0.0484*
c (h ^-1/2 )	-0.3582	-0.3779	-0.2637	-0.6171	0.1226	0.2587
L (h)	1.606	1.819	0.608	1.921	6.4236	0.7480
GP 1h	0.87	10.05	7.16	22.45	3.33	0.1447
GP 2h	1.13	52.33	24.11	71.70	11.08	0.1309
GP 3h	14.69	110.15	53.76	149.95	19.08	0.0755
GP 4h	42.08	184.29	90.13	243.29	27.31	0.0499*
GP 5h	81.83	250.87	116.29	344.51	35.40	0.0456*
GP 6h	118.53	318.83	151.14	412.57	40.81	0.0483*
GP 7h	154.97	366.62	180.62	463.03	40.81	0.0605
GP 8h	187.46	407.55	208.96	507.66	49.39	0.0776
GP 9h	215.29	445.41	231.47	536.39	51.61	0.0878
GP 10h	246.82	484.90	268.86	563.71	55.60	0.1312
GP 11h	276.96	511.67	304.41	587.56	59.33	0.1903
GP 12h	302.52	543.10	335.27	609.86	62.18	0.2312
GP 13h	333.40	565.78	364.99	631.27	65.86	0.3033
GP 14h	360.53	596.52	395.24	652.14	69.35	0.3609
GP 15h	390.61	617.89	422.78	674.69	72.93	0.4287
GP 16h	419.15	634.37	454.33	695.40	76.71	0.5090

vertical_align_top file_download show_chart

more_horizclose
- image
- translate
- link
- article
- vertical_align_top
- file_download
- show_chart
- image
- translate
- link
- article

location_on

Universidade Federal de Minas Gerais, Escola de Veterinária Caixa Postal 567, 30123-970 Belo Horizonte MG - Brazil, Tel.: (55 31) 3409-2041, Tel.: (55 31) 3409-2042 - Belo Horizonte - MG - Brazil
E-mail: abmvz.artigo@gmail.com

rss_feed Acompanhe os números deste periódico no seu leitor de RSS

Acessibilidade / Reportar erro

[TFN1] (-) No detectable values; Acetic Aci. (acetic acid); Ac. Pr (propionic acid); Ac. Isop (Isopropionic acid); Ac. But (butyric acid); Ac. Isob (isobutyric acid); Ac. Val (valeric acid); Ac Lat. (latic acid); (CTRL: control group; NPYEA: non protected live yeast; PYEA: protected live yeast; COMB: combination NPYEA + PYEA; SEM: stand error mean; C1: CTR versus NPYEA, PYEA and COMB; C2: COMB versus NPYEA and PYEA; C3: NPYEA versus PYEA.