Mammary gland secretion pH and electrolytes in prepartum Mangalarga Marchador mares

Lima, J.T.B.; Winter, I.C.; Florez, C.O.; Torres, L.E.C.; Silva, I.E.; Benitez, A.C.; Wenceslau, R.R.; Leme, F.O. Paes; Maranhão, R.P.A.; Teixeira, R.B.C.

doi:10.1590/1678-4162-12480

J.T.B. Lima

Graduate, Universidade Federal de Minas Gerais, Escola de Veterinária, Belo Horizonte, MG, Brasil

https://orcid.org/0000-0003-3866-9247

I.C. Winter

Graduate, Universidade Federal de Minas Gerais, Escola de Veterinária, Belo Horizonte, MG, Brasil

https://orcid.org/0000-0001-9002-5532

C.O. Florez

Graduate, Universidade Federal de Minas Gerais, Escola de Veterinária, Belo Horizonte, MG, Brasil

https://orcid.org/0000-0001-8300-5664

L.E.C. Torres

Graduate, Universidade Federal de Minas Gerais, Escola de Veterinária, Belo Horizonte, MG, Brasil

https://orcid.org/0000-0002-5416-409X

I.E. Silva

Undergraduate, Universidade Federal de Minas Gerais, Escola de Veterinária, Belo Horizonte, MG, Brasil

https://orcid.org/0000-0002-6245-8290

A.C. Benitez

Undergraduate, Universidade Federal de Minas Gerais, Escola de Veterinária, Belo Horizonte, MG, Brasil

https://orcid.org/0000-0002-0562-7051

R.R. Wenceslau

Universidade Federal de Minas Gerais, Escola de Veterinária, Belo Horizonte, MG, Brasil

https://orcid.org/0000-0002-0034-1484

F.O. Paes Leme

Universidade Federal de Minas Gerais, Escola de Veterinária, Belo Horizonte, MG, Brasil

https://orcid.org/0000-0002-4013-0147

R.P.A. Maranhão

Universidade Federal de Minas Gerais, Escola de Veterinária, Belo Horizonte, MG, Brasil

https://orcid.org/0000-0002-2084-672X

R.B.C. Teixeira ^**Corresponding author: teixeiraraffa@gmail.com

Universidade Federal de Viçosa, UFV, Viçosa, MG, Brasil

https://orcid.org/0000-0002-8104-6830

*Corresponding author: teixeiraraffa@gmail.com

Figures (3)
Tables (2)

Thumbnail

Figure 1
pH mammary gland secretion obtained by pH meter and pH paper strip during the last 10 days prior to parturition in Mangalarga Marchador mares. Different letters represent significant statistical difference between time points within the same method of analysis. Time points: T1: ≤ 24hours prior to foaling, T2: 2 days prior to foaling, T3: 3 days prior to foaling, T4:4 days prior to foaling, T5: 5 days prior to foaling, T6: 6 days prior to foaling, T7: 7 days prior to foaling, T8: 8 days prior to foaling, T9: 9 days prior to foaling, T10: 10 days prior to foaling (*p<0.05; ** p<0.0001).

Thumbnail

Figure 2
Bland-Altman graphic for pH measurements obtained by pH meter and pH paper strip in the mammary gland secretion of Mangalarga Marchador mares in the last 10 days prior to parturition. A higher discrepancy between values was noted when lower pH values were observed

Thumbnail

Figure 3
Magnesium, Calcium and Chloride concentrations in the mammary gland secretion of Mangalarga Marchador mares within 10 days prior to foaling. Different letters represent significant statistical difference between time points within the same method of analysis. Mg²⁺= Magnesium, Ca²⁺= Calcium, Cl^-= Chloride. Time points: T1: ≤ 24hours prior to foaling, T2: 2 days prior to foaling, T3: 3 days prior to foaling, T4:4 days prior to foaling, T5: 5 days prior to foaling, T6: 6 days prior to foaling, T7: 7 days prior to foaling, T8: 8 days prior to foaling, T9: 9 days prior to foaling, T10: 10 days prior to foaling (*p<0.05; ** p<0.0001).

Thumbnail

Table 1
Means and standard deviation (SD) for pH and electrolytes during the last 10 days prior to parturition in the mammary gland secretion of Mangalarga Marchador mares

Thumbnail

Table 2
Sensitivity, Specificity, Positive Predict Value (PPV), Negative Predict Value (NPV) and Accuracy for pH, Magnesium (Mg²⁺) and Calcium (Ca²⁺) in the mammary gland secretion of MM mares 24 hours before parturition, considering different threshold values

	pH			Electrolytes mmol/L
	pH meter	pH strip	Ca²⁺	Mg²⁺	Cl^-	K⁺	Na⁺
T1	6.67±0.42^a	6.23±0.53 ^a	12.6±5.8 ^a	10.93±3.66 ^a	96.8±26.25 ^a	17.26±1.88 ^a	69.71±31.36 ^a
T2	6.98±0.45 ^b	6.59±0.59 ^b	8.07±4.14 ^b	7.97±3.0 ^b	86.4±11.7 ^a	18.52±5.31 ^a	70.57±45.74 ^a
T3	7.2±0.37 ^c	6.84±0.54 ^c	4.2±2.77 ^c	7.04±3.14 ^b	88.8±11.89 ^a	17.38±5.94 ^a	81±36.62 ^a
T4	7.3±0.3 ^c	7±0.49 ^c	4.92±3.51 ^c	6.54±2.93 ^b	105.5±44.41 ^a	16.18±3.76 ^a	84.5±36.29 ^a
T5	7.3±0.31 ^c	7.09±0.44 ^c	4.1±3.19 ^c	6.71±3.12 ^b	85.3±8.07 ^a	15.57±4.19 ^a	81.81±34.35 ^a
T6	7.27±0.41 ^c	7.17±0.4 ^d	4.12±3.59 ^c	5.84±3.0 ^b	92.9±11.77 ^a	21.94±28.87 ^a	92.36±34.63 ^a
T7	7.4±0.33 ^c	7.28±0.29 ^d	3.92±3.35 ^c	6.11±2.59 ^b	93.1±11.74 ^a	13.14±4.87 ^a	99.92±39.05 ^a
T8	7.52±0.26 ^c	7.29±0.3 ^d	2.52±1.44 ^c	3.59±1.38 ^b	101±17.29 ^a	13.22±3.49 ^a	97.75±38.55 ^a
T9	7.32±0.2 ^bc	7.24±0.23 ^d	2.52±1.94 ^c	5.23±1.87 ^b	161.6±103.35 ^b	11.57±5.32 ^a	89.67±22.48 ^a
T10	7.32±0.18 ^bc	7.28±0.19 ^d	2.92±4.07 ^c	3.73±2.59 ^b	160.4±101.88 ^b	10.5±5.8 ^a	101±32.19 ^a

	pH						Electrolytes (mmol/L)
	pH meter			pH strip			Mg²⁺	Ca²⁺
VALUES	6.6	6.8*	7	6.2	6.6*	6.8	8.40*	9.79*
Sensitivity (%)	50	65	90	44	86	94	88	90
Specificity (%)	97	93	72	94	76	63	86	95
PPV (%)	84	70	43	64	84	35	65	81
NPV (%)	89	91	96	89	97	98	96	97
Accuracy (%)	88	87	75	85	78	69	87	94

[TFN1] Means with different letters differ from one another within the same column (P<0.05). Mg²⁺= Magnesium, Ca²⁺= Calcium, Cl^-= Chloride, K⁺= Potassium, Na⁺= Sodium. Time points: T1: ≤ 24hours prior to foaling, T2: 2 days prior to foaling, T3: 3 days prior to foaling, T4:4 days prior to foaling, T5: 5 days prior to foaling, T6: 6 days prior to foaling, T7: 7 days prior to foaling, T8: 8 days prior to foaling, T9: 9 days prior to foaling, T10: 10 days prior to foaling.

Mammary gland secretion pH and electrolytes in prepartum Mangalarga Marchador mares

[Concentrações de pH e eletrólitos na secreção da glândula mamária de éguas Mangalarga Marchador no período pré-parto]

ABSTRACT