Experiments on rose micropropagation showed that the root stock Rosa 'Shafter' was an excellent material fast and easily propagated. It has taken 12 months to reach more than a thousand plantlets from a few inoculated meristems. In a first part of the experiments, the meristem culture evolution was observed in the medium Pierik added with 2 mg/l 6-benzylaminopurine, 20 g/l sucrose, 6.4 g/l agar and the organic compounds: 100 mg/l myo-inositol, 0.5 mg/l nicotinic acid, 0S mg/l pyridoxine HCl, 1 mg/l thiamine HCl, 0.5 mg/l folic acid, 0.5 mg/l riboflavin, 1 mg/l calcium pantothenate. In a second part, three experiments were established to observe the rooting required conditions. Roots can be obtained in a liquid medium, with or without auxins, and in a solid medium, with 6.4 g/l agar, with the auxins 0.05 mg/l indolbutiric acid and 0.1 mg/l naftaleneacetic acid.
rose; Rosa 'Shafter'; in vitro micropropagation; root-stock; in vitro rooting; meristem