Identification of citrus species by means of enzymatic polymorphismThe genetic diversity of citrus cultivars was studied by polyacrylamide gel electrophoresis on sweet orange (C. sinensis); tangerines (C. clementine, C. sunki, C. reshni, C. reticulata); Palestine lime (C. Iimettioides); West Indian lime (C. aurantifolia); Rangpur lime (C. limonia), Sour orange (C. aurantium) and Poncirus trifoliata. Citrus leaf extracts were analysed for isozymes of malato dehidrogenase (MDH), malic enzyme (ME), leucine aminopeptidase (LAP), glutamate oxaloacetate transaminase (GOT), phosphoglucose isomerase (PGI), phosphoglucose mutase (PGM) and isocitrate dehydrogenase (IDH). Interespecific differences were observed; but, none between intraespecific C. sinensis cultivars. Some allozymes were observed in addition to those reffered in the literature on starch gel; e.g., allozymes located next to the known Pgm-1 loci. The PGM system revealed the best differentiation between the cultivars. It showed monomeric proteins and 9 alleles. The MDH system had two loci with 7 alleles; the ME system one locus with 3 alleles; the LAP system one possible locus next to the known Lap-1 and 4 alleles; the GOT two loci for dimeric proteins with 7 alleles; the PGI one locus with 3 alleles; and, the IDH one locus with 4 alleles.
citrus; isozymes; cultivars identification