Long Noncoding RNA AF131217.1 Regulated Coronary Slow Flow-Induced Inflammation Affecting Coronary Slow Flow via KLF4

Jiang, Haibing; Ge, Zhengrong; Zhang, Lijing; Yang, Yi; Zhai, Xueqin; Chen, Zhanxi; Wei, Qing

doi:10.21470/1678-9741-2020-0573

Brasil

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ORIGINAL ARTICLE • Braz. J. Cardiovasc. Surg. 37 (04) • 2022 • https://doi.org/10.21470/1678-9741-2020-0573 linkcopy

Long Noncoding RNA AF131217.1 Regulated Coronary Slow Flow-Induced Inflammation Affecting Coronary Slow Flow via KLF4

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Introduction: This study investigated the correlation between the levels of long noncoding ribonucleic acids (lncRNAs) AF131217.1 and coronary slow flow (CSF).

Methods: A total of 22 patients in the high-sensitivity C-reactive protein (hsCRP) group diagnosed with CSF from January 2018 to December 2018 were enrolled in this study. Coronary flow velocity was determined using the thrombolysis in myocardial infarction frame count (TFC) method. Results: LncRNA AF131217.1 expression in the CSF model was activated. Mean TFC was positively correlated with lncRNA AF131217.1 levels and hsCRP levels. LncRNA AF131217.1 induced inflammation factor levels in the in vitro model. Micro ribonucleic acid (miR)-128-3p is a target spot of lncRNA AF131217.1 on the inflammation in vitro model via Kruppel-like factor (KLF) 4. MiR-128-3p reduced inflammation factor levels (tumor necrosis factor alpha, interleukin [IL]-6, IL-1β, and IL-18). Conclusion: Thus, lncRNA AF131217.1 promoted inflammation in the regulated CSF via KLF4 by miR-128-3p.

Keywords: Myocardial Infarctation; Inflammation; Interleukin-18; Interleukin-6; RNA; Long Noncoding; C-Reactive Protein; MicroRNAs; GKLF protein

Abbreviations, acronyms & symbols
ACS	= Acute coronary syndrome	LncRNAs	= Long noncoding ribonucleic acids
C/EBPβ	= CCAAT/enhancer binding protein beta	LPS	= Lipopolysaccharide
cDNA	= Complementary deoxyribonucleic acid	MiR	= Micro ribonucleic acid
CSF	= Coronary slow flow	mRNA	= Messenger ribonucleic acid
ELISA	= Enzyme-linked immunosorbent assay	Mut	= Mutant
GAPDH	= Glyceraldehyde-3-phosphate dehydrogenase	NF-κB	= Nuclear factor kappa B
hsCRP	= High-sensitivity C-reactive protein	RNA	= Ribonucleic acid
HUVECs	= Human umbilical vein endothelial cells	TFC	= Thrombolysis in myocardial infarction frame count
IL	= Interleukin	TNF-α	= Tumor necrosis factor alpha
KLF	= Kruppel-like factors	WT	= Wild type

Authors' roles & responsibilities
ZG	Substantial contributions to the acquisition of data for the work; revising the work for important intellectual content; final approval of the version to be published
LZ	Substantial contributions to the acquisition of data for the work; final approval of the version to be published
YY	Substantial contributions to the acquisition of data for the work; final approval of the version to be published
XZ	Substantial contributions to the analysis of data for the work; final approval of the version to be published
ZC	Drafting the work and revising it; final approval of the version to be published
QW	Revising the work; final approval of the version to be published
HJ	Substantial contributions to the conception or design of the work; agreement to be accountable for all aspects of the work in ensuring that questions related to the integrity of any part of the work are appropriately investigated and resolved; final approval of the version to be published

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