Virulência de fungos entomopatogênicos sobre larvas de Lepidoptera: Noctuidae

Loureiro, Elisângela de S.; Dias, Pamella M.; Pessoa, Luis G. A.; Amaral, Tatiana S. do; Pessoa, Marcelo B.; Gregori, Gabriella S. de; Rodrigues, Andressa S.; Godoy, Maurício S. de

doi:10.1590/1983-21252024v3712375rc

Brasil

home Sumário navigate_before Anterior Atual Seguinte navigate_next

home Sumário

Scientific Article • Rev. Caatinga 37 • 2024 • https://doi.org/10.1590/1983-21252024v3712375rc linkcopiar

Virulência de fungos entomopatogênicos sobre larvas de Lepidoptera: Noctuidae

Autoria SCIMAGO INSTITUTIONS RANKINGS

RESUMO

Atualmente, os sistemas de cultivo agrícola adotam o manejo integrado de pragas (MIP) como modelo de sucesso para o controle de pragas. O uso de fungos entomopatogênicos tem aumentado no manejo integrado devido ao seu grande potencial em reduzir as populações de pragas agrícolas sem causar danos à saúde humana e aos ecossistemas. Beauveria bassiana e Metarhizium anisopliae são as espécies de fungos mais utilizadas no controle biológico, contando com muitos isolados comercializados no mundo. Helicoverpa armigera é uma praga de importância agrícola, causadora de prejuízos associados a diversas culturas. Assim, esse estudo teve como objetivo avaliar o potencial virulento de Beauveria bassiana, Metarhizium anisopliae e Metarhizium rileyi no controle biológico dos estágios larvais de H. armigera. Os resultados indicaram que em diferentes concentrações (1 × 10⁷, 1 × 10⁸ e 1 × 10⁹ con.mL^-1) e métodos de aplicação (DA ou DF) B. bassiana (cepa ESALQ PL63), M. anisopliae (cepa ESALQ E9) e M. rileyi (cepa UFMS 03) causaram baixa mortalidade e não foram virulentos ao primeiro, terceiro e quinto ínstares de H. armigera. A mortalidade pelo tratamento com B. bassiana (cepa ESALQ PL63) foi confirmada por conidiogênese em todos os cadáveres. Dada a importância de H. armigera como praga agrícola e a biodiversidade de fungos entomopatogênicos no Brasil, novas investigações sobre a virulência de cepas fúngicas são necessárias para aprimorar o manejo integrado de pragas através do controle microbiano, o potencial para novas cepas e o entendimento das relações entre microrganismos e os mecanismos de defesa dos hospedeiros.

Palavras-chave Controle biológico; Beauveria; Metarhizium; Helicoverpa. Manejo de pragas.

Source of variation	DF	3 DAA	6 DAA	9 DAA	12 DAA
Product (P)	3	0.50 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.52 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.62 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.75 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.
Application method (A)	1	0.89 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.88 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.75 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.
Concentration (C)	3	0.95 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.82 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.99 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.98 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.
A×P	3	0.88 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.93 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.99 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.94 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.
A×C	3	0.99 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.99 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.
P×C	9	0.95 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.99 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.99 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.98 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.
A×P×C	9	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.99 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.
Residue	580	62.36	0.07	0.06	0.06

Product (P)	3 DAA	6 DAA	9 DAA	12 DAA
Control	0.03± 0.03 a	0.03±0.03 a	0.03±0.03 a	0.03±0.03 a
B. bassiana	0.09±0.02 a	0.09±0.02 a	0.08±0.02 a	0.07±0.02 a
M. anisopliae	0.07±0.02 a	0.07±0.01 a	0.06±0.02 a	0.06±0.02 a
M. rileyi	0.08±0.02 a	0.08±0.02 a	0.06±0.02 a	0.07±0.02 a
Application method (A)	3 DAA	6 DAA	9 DAA	12 DAA
Direct	0.08±0.01 a	0.08±0.01 a	0.06±0.01 a	0.07±0.01 a
Dry Film	0.08±0.01 a	0.08±0.01 a	0.07±0.01 a	0.06±0.01 a
Concentration (C)	3 DAA	6 DAA	9 DAA	12 DAA
10⁷ conidia mL^-1	0.09±0.02 a	0.08±0.02 a	0.07±0.02 a	0.06±0.02 a
10⁸ conidia mL^-1	0.09±0.02 a	0.07±0.02 a	0.06±0.02 a	0.06±0.02 a
10⁹ conidia mL^-1	0.08±0.02 a	0.10±0.02 a	0.07±0.02 a	0.07±0.02 a

Source of variation	DF	3 DAA	6 DAA	9 DAA	12 DAA
Product (P)	3	0.43 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.50 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.62 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.70 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.
Application method (A)	1	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.87 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.
Concentration (C)	3	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.97 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.98 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.94 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.
P×A	3	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.98 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.98 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.
P×C	9	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.
A×C	3	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.
P×A×C	9	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.
Residue	580	0.08	0.07	0.06	0.06

Product (P)	3 DAA	6 DAA	9 DAA	12 DAA
Control	0.03±0.00 a	0.03±0.00 a	0.03±0.00 a	0.03±0.00 a
B. bassiana	0.10±0.00 a	0.09±0.00 a	0.08±0.00 a	0.07±0.00 a
M. anisopliae	0.10±0.00 a	0.07±0.00 a	0.06±0.00 a	0.07±0.00 a
M. rileyi	0.10±0. 00 a	0.08±0.00 a	0.06±0.00 a	0.07±0. 00 a
Application method (A)	3 DAA	6 DAA	9 DAA	12 DAA
Direct	0.09±0.00 a	0.08±0.00 a	0.06±0.00 a	0.07±0.00 a
Dry film	0.09±0. 00 a	0.08±0.00 a	0.07±0.00 a	0.07±0. 00 a
Concentration (C)	3 DAA	6 DAA	9 DAA	12 DAA
10⁷ conidia mL^-1	0.10±0.00 a	0.08±0.00 a	0.03±0.00 a	0.07±0.00 a
10⁸ conidia mL^-1	0.10±0.00 a	0.08±0.00 a	0.07±0.00 a	0.06±0.00 a
10⁹ conidia mL^-1	0.10±0.00 a	0.09±0.00 a	0.06±0.00 a	0.08±0.00 a

Source of variation	DF	3 DAA	6 DAA	9 DAA	12 DAA
Products (P)	3	0.54 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.23 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.27 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.35 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.
Application method (A)	1	0.63 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.76 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.76 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.87 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.
Concentration (C)	3	0.94 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.80 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.89 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.93 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.
P×A	3	0.98 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.88 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.94 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.85 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.
P×C	9	0.99 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.99 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.99 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.
A×C	3	0.98 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	0.95 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.
P×A×C	9	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.	1.00 ^ns ns Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.
Residue	580	0.06	0.07	0.07	0.06

Product (P)	3 DAA	6 DAA	9 DAA	12 DAA
Control	0.03±0.00 a	0.03±0.00 a	0.03±0.00 a	0.03±0.00 a
B. bassiana	0.08±0.00 a	0.10±0.00 a	0.10±0.00 a	0.09±0.00 a
M. anisopliae	0.07±0.00 a	0.08±0.00 a	0.07±0.00 a	0.05±0.00 a
M. rileyi	0.06±0. 00 a	0.06±0.00 a	0.06±0.00 a	0.07±0.00 a
Application method (A)	3 DAA	6 DAA	9 DAA	12 DAA
Direct	0.07±0.00 a	0.08±0.00 a	0.08±0.00 a	0.06±0.00 a
Dry film	0.06±0.00 a	0.07±0.00 a	0.07±0.00 a	0.07±0.00 a
Concentration (C)	3 DAA	6 DAA	9 DAA	12 DAA
10⁷ conidia mL^-1	0.08±0.00 a	0.06±0.00 a	0.07±0.00 a	0.07±0.00 a
10⁸ conidia mL^-1	0.08±0.00 a	0.09±0.00 a	0.09±0.00 a	0.08±0.00 a
10⁹ conidia mL^-1	0.07±0.00 a	0.08±0.00 a	0.08±0.00 a	0.06±0.00 a

vertical_align_top file_download show_chart

more_horizclose
- image
- translate
- link
- article
- vertical_align_top
- file_download
- show_chart
- image
- translate
- link
- article

location_on

Universidade Federal Rural do Semi-Árido Avenida Francisco Mota, número 572, Bairro Presidente Costa e Silva, Cep: 5962-5900, Telefone: 55 (84) 3317-8297 - Mossoró - RN - Brazil
E-mail: caatinga@ufersa.edu.br

rss_feed Acompanhe os números deste periódico no seu leitor de RSS

Acessibilidade / Reportar erro

[TFN1] ^*
Significant at a 5% level by the F test;

[TFN2] ^ns
Not significant. DF = degrees of freedom. Bioassay conducted under laboratory conditions: air temperature of 25±1 °C, relative air humidity of 70±10%, and 12-hour photoperiod.